The Aspergillus nidulans cetA and calA genes are involved in conidial germination and cell wall morphogenesis.
Identifieur interne : 000335 ( Main/Exploration ); précédent : 000334; suivant : 000336The Aspergillus nidulans cetA and calA genes are involved in conidial germination and cell wall morphogenesis.
Auteurs : Ravit Belaish [Israël] ; Haim Sharon ; Emma Levdansky ; Shulamit Greenstein ; Yana Shadkchan ; Nir OsherovSource :
- Fungal genetics and biology : FG & B [ 1096-0937 ] ; 2008.
Descripteurs français
- KwdFr :
- Aspergillus nidulans (croissance et développement), Aspergillus nidulans (génétique), Aspergillus nidulans (métabolisme), Modèles génétiques (MeSH), Morphogenèse (MeSH), Mutation (MeSH), Paroi cellulaire (métabolisme), Phénotype (MeSH), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Régulation de l'expression des gènes fongiques (MeSH), Spores fongiques (croissance et développement), Spores fongiques (génétique), Technique de Northern (MeSH), Technique de Western (MeSH).
- MESH :
- croissance et développement : Aspergillus nidulans, Spores fongiques.
- génétique : Aspergillus nidulans, Protéines fongiques, Spores fongiques.
- métabolisme : Aspergillus nidulans, Paroi cellulaire, Protéines fongiques.
- Modèles génétiques, Morphogenèse, Mutation, Phénotype, Régulation de l'expression des gènes fongiques, Technique de Northern, Technique de Western.
English descriptors
- KwdEn :
- Aspergillus nidulans (genetics), Aspergillus nidulans (growth & development), Aspergillus nidulans (metabolism), Blotting, Northern (MeSH), Blotting, Western (MeSH), Cell Wall (metabolism), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Fungal (MeSH), Models, Genetic (MeSH), Morphogenesis (MeSH), Mutation (MeSH), Phenotype (MeSH), Spores, Fungal (genetics), Spores, Fungal (growth & development).
- MESH :
- chemical , genetics : Fungal Proteins.
- genetics : Aspergillus nidulans, Spores, Fungal.
- growth & development : Aspergillus nidulans, Spores, Fungal.
- metabolism : Aspergillus nidulans, Cell Wall, Fungal Proteins.
- Blotting, Northern, Blotting, Western, Gene Expression Regulation, Fungal, Models, Genetic, Morphogenesis, Mutation, Phenotype.
Abstract
The Aspergillus nidulans genes cetA (AN3079.2) and calA (AN7619.2) encode a novel class of fungal thaumatin-like proteins of unknown function. Deletion of cetA does not result in an observable phenotype [Greenstein, S., Shadkchan, Y., Jadoun, J., Sharon, C., Markovich, S., Osherov, N., 2006. Analysis of the Aspergillus nidulans thaumatin-like cetA gene and evidence for transcriptional repression of pyr4 expression in the cetA-disrupted strain. Fungal Genet. Biol. 43, 42-53]. We prepared knockout calA and calA/cetA A. nidulans strains. The calA mutants were phenotypically identical to the wild-type. In contrast, the cetA/calA double mutant showed a synthetic lethal phenotype suggesting that the two genes affect a single function or pathway: most of its conidia were completely inhibited in germination. Many collapsed and underwent lysis. A few showed abnormal germination characterized by short swollen hyphae and abnormal hyphal branching. Nongerminated conidia contained a single condensed nucleus suggesting a block in early germination. This is the first functional analysis of the novel cetA/calA family of thaumatin-like genes and their role in A. nidulans conidial germination. We show that CETA and CALA are secreted proteins that together play an essential role in early conidial germination.
DOI: 10.1016/j.fgb.2007.07.005
PubMed: 17703972
Affiliations:
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uniqKey="Levdansky E" first="Emma" last="Levdansky">Emma Levdansky</name></author><author><name sortKey="Greenstein, Shulamit" sort="Greenstein, Shulamit" uniqKey="Greenstein S" first="Shulamit" last="Greenstein">Shulamit Greenstein</name></author><author><name sortKey="Shadkchan, Yana" sort="Shadkchan, Yana" uniqKey="Shadkchan Y" first="Yana" last="Shadkchan">Yana Shadkchan</name></author><author><name sortKey="Osherov, Nir" sort="Osherov, Nir" uniqKey="Osherov N" first="Nir" last="Osherov">Nir Osherov</name></author></analytic><series><title level="j">Fungal genetics and biology : FG & B</title><idno type="eISSN">1096-0937</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aspergillus nidulans (genetics)</term><term>Aspergillus nidulans (growth & development)</term><term>Aspergillus nidulans (metabolism)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Cell Wall (metabolism)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Models, Genetic (MeSH)</term><term>Morphogenesis (MeSH)</term><term>Mutation (MeSH)</term><term>Phenotype (MeSH)</term><term>Spores, Fungal (genetics)</term><term>Spores, Fungal (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aspergillus nidulans (croissance et développement)</term><term>Aspergillus nidulans (génétique)</term><term>Aspergillus nidulans (métabolisme)</term><term>Modèles génétiques (MeSH)</term><term>Morphogenèse (MeSH)</term><term>Mutation (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Phénotype (MeSH)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Régulation de l'expression des gènes fongiques (MeSH)</term><term>Spores fongiques (croissance et développement)</term><term>Spores fongiques (génétique)</term><term>Technique de Northern (MeSH)</term><term>Technique de Western (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Aspergillus nidulans</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aspergillus nidulans</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Aspergillus nidulans</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aspergillus nidulans</term><term>Protéines fongiques</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Aspergillus nidulans</term><term>Cell Wall</term><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aspergillus nidulans</term><term>Paroi cellulaire</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Northern</term><term>Blotting, Western</term><term>Gene Expression Regulation, Fungal</term><term>Models, Genetic</term><term>Morphogenesis</term><term>Mutation</term><term>Phenotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Modèles génétiques</term><term>Morphogenèse</term><term>Mutation</term><term>Phénotype</term><term>Régulation de l'expression des gènes fongiques</term><term>Technique de Northern</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Aspergillus nidulans genes cetA (AN3079.2) and calA (AN7619.2) encode a novel class of fungal thaumatin-like proteins of unknown function. Deletion of cetA does not result in an observable phenotype [Greenstein, S., Shadkchan, Y., Jadoun, J., Sharon, C., Markovich, S., Osherov, N., 2006. Analysis of the Aspergillus nidulans thaumatin-like cetA gene and evidence for transcriptional repression of pyr4 expression in the cetA-disrupted strain. Fungal Genet. Biol. 43, 42-53]. We prepared knockout calA and calA/cetA A. nidulans strains. The calA mutants were phenotypically identical to the wild-type. In contrast, the cetA/calA double mutant showed a synthetic lethal phenotype suggesting that the two genes affect a single function or pathway: most of its conidia were completely inhibited in germination. Many collapsed and underwent lysis. A few showed abnormal germination characterized by short swollen hyphae and abnormal hyphal branching. Nongerminated conidia contained a single condensed nucleus suggesting a block in early germination. This is the first functional analysis of the novel cetA/calA family of thaumatin-like genes and their role in A. nidulans conidial germination. We show that CETA and CALA are secreted proteins that together play an essential role in early conidial germination.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17703972</PMID><DateCompleted><Year>2008</Year><Month>03</Month><Day>06</Day></DateCompleted><DateRevised><Year>2008</Year><Month>02</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0937</ISSN><JournalIssue CitedMedium="Internet"><Volume>45</Volume><Issue>3</Issue><PubDate><Year>2008</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Fungal genetics and biology : FG & B</Title><ISOAbbreviation>Fungal Genet Biol</ISOAbbreviation></Journal><ArticleTitle>The Aspergillus nidulans cetA and calA genes are involved in conidial germination and cell wall morphogenesis.</ArticleTitle><Pagination><MedlinePgn>232-42</MedlinePgn></Pagination><Abstract><AbstractText>The Aspergillus nidulans genes cetA (AN3079.2) and calA (AN7619.2) encode a novel class of fungal thaumatin-like proteins of unknown function. Deletion of cetA does not result in an observable phenotype [Greenstein, S., Shadkchan, Y., Jadoun, J., Sharon, C., Markovich, S., Osherov, N., 2006. Analysis of the Aspergillus nidulans thaumatin-like cetA gene and evidence for transcriptional repression of pyr4 expression in the cetA-disrupted strain. Fungal Genet. Biol. 43, 42-53]. We prepared knockout calA and calA/cetA A. nidulans strains. The calA mutants were phenotypically identical to the wild-type. In contrast, the cetA/calA double mutant showed a synthetic lethal phenotype suggesting that the two genes affect a single function or pathway: most of its conidia were completely inhibited in germination. Many collapsed and underwent lysis. A few showed abnormal germination characterized by short swollen hyphae and abnormal hyphal branching. Nongerminated conidia contained a single condensed nucleus suggesting a block in early germination. This is the first functional analysis of the novel cetA/calA family of thaumatin-like genes and their role in A. nidulans conidial germination. We show that CETA and CALA are secreted proteins that together play an essential role in early conidial germination.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Belaish</LastName><ForeName>Ravit</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Ramat-Aviv 69978, Tel-Aviv, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sharon</LastName><ForeName>Haim</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Levdansky</LastName><ForeName>Emma</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Greenstein</LastName><ForeName>Shulamit</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Shadkchan</LastName><ForeName>Yana</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Osherov</LastName><ForeName>Nir</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2007</Year><Month>07</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Fungal Genet Biol</MedlineTA><NlmUniqueID>9607601</NlmUniqueID><ISSNLinking>1087-1845</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001233" MajorTopicYN="N">Aspergillus nidulans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008957" MajorTopicYN="N">Models, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009024" MajorTopicYN="N">Morphogenesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2007</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2007</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2007</Year><Month>07</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>8</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>3</Month><Day>7</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>8</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17703972</ArticleId><ArticleId IdType="pii">S1087-1845(07)00122-3</ArticleId><ArticleId IdType="doi">10.1016/j.fgb.2007.07.005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Israël</li></country></list><tree><noCountry><name sortKey="Greenstein, Shulamit" sort="Greenstein, Shulamit" uniqKey="Greenstein S" first="Shulamit" last="Greenstein">Shulamit Greenstein</name><name sortKey="Levdansky, Emma" sort="Levdansky, Emma" uniqKey="Levdansky E" first="Emma" last="Levdansky">Emma Levdansky</name><name sortKey="Osherov, Nir" sort="Osherov, Nir" uniqKey="Osherov N" first="Nir" last="Osherov">Nir Osherov</name><name sortKey="Shadkchan, Yana" sort="Shadkchan, Yana" uniqKey="Shadkchan Y" first="Yana" last="Shadkchan">Yana Shadkchan</name><name sortKey="Sharon, Haim" sort="Sharon, Haim" uniqKey="Sharon H" first="Haim" last="Sharon">Haim Sharon</name></noCountry><country name="Israël"><noRegion><name sortKey="Belaish, Ravit" sort="Belaish, Ravit" uniqKey="Belaish R" first="Ravit" last="Belaish">Ravit Belaish</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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